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eb3 gfp  (Oxford Instruments)
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Oxford Instruments eb3 gfp
Reduction in DCX destabilizes microtubules in vitro. A–F, Microtubule stability in cerebellar neurons. A, C, E, Transfected neurons are labeled with GFP (green), immunostained with antidetyrosinated tubulin antibodies (red), and the nuclei labeled with DAPI (blue). B, D, F, Colocalization between GFP and detyrosinated tubulin is shown in a false purple color. The treatments include control shRNA (A, B), DCX shRNA (C, D), and a combination of DCX and MARK2 shRNA (E, F). Scale bar, F, 10 μm. G, Quantification of Pearson's correlation of the colocalization between GFP and detyrosinated tubulin was calculated using Imaris colocalization measurement tool and subjected to ANOVA statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001; error bars indicate ±SEM. H, Hippocampal neurons transfected with indicated shRNA and <t>EB3-GFP</t> tracked using Imaris cell tracking system. A representative neuron with lines shown in different colors marking the advance of individual tracks over time. Scale bar, H, 5 μm.
Eb3 Gfp, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Reduction in DCX destabilizes microtubules in vitro. A–F, Microtubule stability in cerebellar neurons. A, C, E, Transfected neurons are labeled with GFP (green), immunostained with antidetyrosinated tubulin antibodies (red), and the nuclei labeled with DAPI (blue). B, D, F, Colocalization between GFP and detyrosinated tubulin is shown in a false purple color. The treatments include control shRNA (A, B), DCX shRNA (C, D), and a combination of DCX and MARK2 shRNA (E, F). Scale bar, F, 10 μm. G, Quantification of Pearson's correlation of the colocalization between GFP and detyrosinated tubulin was calculated using Imaris colocalization measurement tool and subjected to ANOVA statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001; error bars indicate ±SEM. H, Hippocampal neurons transfected with indicated shRNA and EB3-GFP tracked using Imaris cell tracking system. A representative neuron with lines shown in different colors marking the advance of individual tracks over time. Scale bar, H, 5 μm.

Journal: The Journal of Neuroscience

Article Title: Antagonistic Effects of Doublecortin and MARK2/Par-1 in the Developing Cerebral Cortex

doi: 10.1523/JNEUROSCI.2363-08.2008

Figure Lengend Snippet: Reduction in DCX destabilizes microtubules in vitro. A–F, Microtubule stability in cerebellar neurons. A, C, E, Transfected neurons are labeled with GFP (green), immunostained with antidetyrosinated tubulin antibodies (red), and the nuclei labeled with DAPI (blue). B, D, F, Colocalization between GFP and detyrosinated tubulin is shown in a false purple color. The treatments include control shRNA (A, B), DCX shRNA (C, D), and a combination of DCX and MARK2 shRNA (E, F). Scale bar, F, 10 μm. G, Quantification of Pearson's correlation of the colocalization between GFP and detyrosinated tubulin was calculated using Imaris colocalization measurement tool and subjected to ANOVA statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001; error bars indicate ±SEM. H, Hippocampal neurons transfected with indicated shRNA and EB3-GFP tracked using Imaris cell tracking system. A representative neuron with lines shown in different colors marking the advance of individual tracks over time. Scale bar, H, 5 μm.

Article Snippet: H , Hippocampal neurons transfected with indicated shRNA and EB3-GFP tracked using Imaris cell tracking system.

Techniques: In Vitro, Transfection, Labeling, Control, shRNA, Cell Tracking Assay